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illumina nextseq 550 platform  (Illumina Inc)


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    Structured Review

    Illumina Inc illumina nextseq 550 platform
    Illumina Nextseq 550 Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 7261 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nextseq/NextSeq+550+Sequencing+System/pmc13682369-138-5-5
    Average 99 stars, based on 7261 article reviews
    illumina nextseq 550 platform - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title: Geographical Variation in Antimalarial Drug Resistance Marker Prevalence across the Southern African Elimination Eight Region
    Article Snippet: Amplicon-based sequencing was performed using the MAD 4 HatTeR (Multiplex Amplicons for Drug, Diagnostic, Diversity, and Differentiation Haplotypes using Targeted Resequencing) multiplex panel and protocol, an adaptation of the CleanPlex Amplicon Sequencing Technology (Paragon Genomics, Inc., Hayward, CA, USA), using the MAD 4 HatTeR primer pools D1.1, R1.1 or R1.2, and R2.1. .. Sequencing was conducted on Illumina MiSeq, NextSeq, or NovaSeq instruments (Illumina, Inc., San Diego, CA, USA) using 150 paired-end reads. ..

    Article Title: A prospective pilot study of urine microRNA as a biomarker of urinary radiation toxicity following MRI-guided prostate stereotactic body radiotherapy
    Article Snippet: miRNA sequencing libraries were prepared from 10 ng of input RNA using the QIAseq miRNA Library Kit (QIAGEN). .. In addition to standard adapters, unique molecular identifiers (UMI) were added at the reverse transcription step before 19 cycles of amplification by PCR and sequencing on a NextSeq (Illumina Inc.) sequencing instrument. ..

    Article Title: MYB activity drives emergent enhancer activation and enhancer-promoter interactions in acute lymphoblastic leukemia.
    Article Snippet: Medical Research Council Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford, United Kingdom; Oxford Translational Myeloma Centre, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Oxford, United Kingdom; Department of Haematology, Oxford University Hospitals NHS Trust, Oxford, United Kingdom; Dark Blue Therapeutics Ltd, Oxford, United Kingdom; Radcliffe Department of Medicine, Oxford National Institute of Health Research Biomedical Research Centre, University of Oxford, Oxford, United Kingdom; and Hugh and Josseline Langmuir Centre for Myeloma Research, Centre for Haematology, Department of Immunology and Inflammation, Imperial College London, London, United Kingdom

    Article Title: Distinct genomic landscapes of mismatch-repair deficiency (dMMR)/microsatellite instability-high (MSI-H) gastrointestinal (GI) cancer depending on tumor mutation burden (TMB) levels.
    Article Snippet: A total of 2272 tumor samples collected worldwide by a commercial CLIA- certified laboratory database (GI tumors, N=48 606; Caris Life Sciences, Phoenix, USA) (referred to as CARIS) were defined as dMMR/MSI- H by a combination of multiple test platforms, including fragment analysis (FA, Promega, Madison, Wisconsin, USA), immunohistochemistry staining (IHC, MLH1, M1 antibody; MSH2, G2191129 antibody; MSH6, 44 antibody; and PMS2, EPR3947 antibody (Ventana Medical Systems, Tucson, Arizona, USA)), and NGS (7000 target microsatellite loci were examined and compared with the reference genome hg19 from the University of California).12 In the rare cases of discordant results, the MSI or MMR status of the tumor was determined in the order of IHC, FA, and NGS. .. NGS was performed on genomic DNA isolated from formalin- fixed paraffin- embedded (FFPE) tumor samples using the NextSeq or NovaSeq 6000 platforms (Illumina, San Diego, California, USA).13 All variants were detected with >99% confidence based on allele frequency and amplicon coverage, with an average sequencing depth of coverage of >500 and an analytic sensitivity of 5%. ..

    Reverse Transcription:

    Article Title: A prospective pilot study of urine microRNA as a biomarker of urinary radiation toxicity following MRI-guided prostate stereotactic body radiotherapy
    Article Snippet: miRNA sequencing libraries were prepared from 10 ng of input RNA using the QIAseq miRNA Library Kit (QIAGEN). .. In addition to standard adapters, unique molecular identifiers (UMI) were added at the reverse transcription step before 19 cycles of amplification by PCR and sequencing on a NextSeq (Illumina Inc.) sequencing instrument. ..

    Article Title: Parasympathetic nervous activity and CTRA gene expression among healthy young adults in Japan
    Article Snippet: .. Briefly, RNA was extracted (Qiagen RNeasy), tested for suitable mass (RiboGreen RNA) and integrity (Agilent TapeStation), converted to cDNA using a high-efficiency mRNA targeted reverse transcription system (Lexogen QuantSeq 3′ FWD), and sequenced on an Illumina NextSeq instrument by Lexogen Services GmbH, all following the manufacturer's standard protocols. ..

    Amplification:

    Article Title: A prospective pilot study of urine microRNA as a biomarker of urinary radiation toxicity following MRI-guided prostate stereotactic body radiotherapy
    Article Snippet: miRNA sequencing libraries were prepared from 10 ng of input RNA using the QIAseq miRNA Library Kit (QIAGEN). .. In addition to standard adapters, unique molecular identifiers (UMI) were added at the reverse transcription step before 19 cycles of amplification by PCR and sequencing on a NextSeq (Illumina Inc.) sequencing instrument. ..

    Article Title: Distinct genomic landscapes of mismatch-repair deficiency (dMMR)/microsatellite instability-high (MSI-H) gastrointestinal (GI) cancer depending on tumor mutation burden (TMB) levels.
    Article Snippet: A total of 2272 tumor samples collected worldwide by a commercial CLIA- certified laboratory database (GI tumors, N=48 606; Caris Life Sciences, Phoenix, USA) (referred to as CARIS) were defined as dMMR/MSI- H by a combination of multiple test platforms, including fragment analysis (FA, Promega, Madison, Wisconsin, USA), immunohistochemistry staining (IHC, MLH1, M1 antibody; MSH2, G2191129 antibody; MSH6, 44 antibody; and PMS2, EPR3947 antibody (Ventana Medical Systems, Tucson, Arizona, USA)), and NGS (7000 target microsatellite loci were examined and compared with the reference genome hg19 from the University of California).12 In the rare cases of discordant results, the MSI or MMR status of the tumor was determined in the order of IHC, FA, and NGS. .. NGS was performed on genomic DNA isolated from formalin- fixed paraffin- embedded (FFPE) tumor samples using the NextSeq or NovaSeq 6000 platforms (Illumina, San Diego, California, USA).13 All variants were detected with >99% confidence based on allele frequency and amplicon coverage, with an average sequencing depth of coverage of >500 and an analytic sensitivity of 5%. ..

    Polymerase Chain Reaction:

    Article Title: A prospective pilot study of urine microRNA as a biomarker of urinary radiation toxicity following MRI-guided prostate stereotactic body radiotherapy
    Article Snippet: miRNA sequencing libraries were prepared from 10 ng of input RNA using the QIAseq miRNA Library Kit (QIAGEN). .. In addition to standard adapters, unique molecular identifiers (UMI) were added at the reverse transcription step before 19 cycles of amplification by PCR and sequencing on a NextSeq (Illumina Inc.) sequencing instrument. ..

    Next-Generation Sequencing:

    Article Title: Distinct genomic landscapes of mismatch-repair deficiency (dMMR)/microsatellite instability-high (MSI-H) gastrointestinal (GI) cancer depending on tumor mutation burden (TMB) levels.
    Article Snippet: A total of 2272 tumor samples collected worldwide by a commercial CLIA- certified laboratory database (GI tumors, N=48 606; Caris Life Sciences, Phoenix, USA) (referred to as CARIS) were defined as dMMR/MSI- H by a combination of multiple test platforms, including fragment analysis (FA, Promega, Madison, Wisconsin, USA), immunohistochemistry staining (IHC, MLH1, M1 antibody; MSH2, G2191129 antibody; MSH6, 44 antibody; and PMS2, EPR3947 antibody (Ventana Medical Systems, Tucson, Arizona, USA)), and NGS (7000 target microsatellite loci were examined and compared with the reference genome hg19 from the University of California).12 In the rare cases of discordant results, the MSI or MMR status of the tumor was determined in the order of IHC, FA, and NGS. .. NGS was performed on genomic DNA isolated from formalin- fixed paraffin- embedded (FFPE) tumor samples using the NextSeq or NovaSeq 6000 platforms (Illumina, San Diego, California, USA).13 All variants were detected with >99% confidence based on allele frequency and amplicon coverage, with an average sequencing depth of coverage of >500 and an analytic sensitivity of 5%. ..

    Isolation:

    Article Title: Distinct genomic landscapes of mismatch-repair deficiency (dMMR)/microsatellite instability-high (MSI-H) gastrointestinal (GI) cancer depending on tumor mutation burden (TMB) levels.
    Article Snippet: A total of 2272 tumor samples collected worldwide by a commercial CLIA- certified laboratory database (GI tumors, N=48 606; Caris Life Sciences, Phoenix, USA) (referred to as CARIS) were defined as dMMR/MSI- H by a combination of multiple test platforms, including fragment analysis (FA, Promega, Madison, Wisconsin, USA), immunohistochemistry staining (IHC, MLH1, M1 antibody; MSH2, G2191129 antibody; MSH6, 44 antibody; and PMS2, EPR3947 antibody (Ventana Medical Systems, Tucson, Arizona, USA)), and NGS (7000 target microsatellite loci were examined and compared with the reference genome hg19 from the University of California).12 In the rare cases of discordant results, the MSI or MMR status of the tumor was determined in the order of IHC, FA, and NGS. .. NGS was performed on genomic DNA isolated from formalin- fixed paraffin- embedded (FFPE) tumor samples using the NextSeq or NovaSeq 6000 platforms (Illumina, San Diego, California, USA).13 All variants were detected with >99% confidence based on allele frequency and amplicon coverage, with an average sequencing depth of coverage of >500 and an analytic sensitivity of 5%. ..

    Formalin-fixed Paraffin-Embedded:

    Article Title: Distinct genomic landscapes of mismatch-repair deficiency (dMMR)/microsatellite instability-high (MSI-H) gastrointestinal (GI) cancer depending on tumor mutation burden (TMB) levels.
    Article Snippet: A total of 2272 tumor samples collected worldwide by a commercial CLIA- certified laboratory database (GI tumors, N=48 606; Caris Life Sciences, Phoenix, USA) (referred to as CARIS) were defined as dMMR/MSI- H by a combination of multiple test platforms, including fragment analysis (FA, Promega, Madison, Wisconsin, USA), immunohistochemistry staining (IHC, MLH1, M1 antibody; MSH2, G2191129 antibody; MSH6, 44 antibody; and PMS2, EPR3947 antibody (Ventana Medical Systems, Tucson, Arizona, USA)), and NGS (7000 target microsatellite loci were examined and compared with the reference genome hg19 from the University of California).12 In the rare cases of discordant results, the MSI or MMR status of the tumor was determined in the order of IHC, FA, and NGS. .. NGS was performed on genomic DNA isolated from formalin- fixed paraffin- embedded (FFPE) tumor samples using the NextSeq or NovaSeq 6000 platforms (Illumina, San Diego, California, USA).13 All variants were detected with >99% confidence based on allele frequency and amplicon coverage, with an average sequencing depth of coverage of >500 and an analytic sensitivity of 5%. ..

    Sensitivity:

    Article Title: Distinct genomic landscapes of mismatch-repair deficiency (dMMR)/microsatellite instability-high (MSI-H) gastrointestinal (GI) cancer depending on tumor mutation burden (TMB) levels.
    Article Snippet: A total of 2272 tumor samples collected worldwide by a commercial CLIA- certified laboratory database (GI tumors, N=48 606; Caris Life Sciences, Phoenix, USA) (referred to as CARIS) were defined as dMMR/MSI- H by a combination of multiple test platforms, including fragment analysis (FA, Promega, Madison, Wisconsin, USA), immunohistochemistry staining (IHC, MLH1, M1 antibody; MSH2, G2191129 antibody; MSH6, 44 antibody; and PMS2, EPR3947 antibody (Ventana Medical Systems, Tucson, Arizona, USA)), and NGS (7000 target microsatellite loci were examined and compared with the reference genome hg19 from the University of California).12 In the rare cases of discordant results, the MSI or MMR status of the tumor was determined in the order of IHC, FA, and NGS. .. NGS was performed on genomic DNA isolated from formalin- fixed paraffin- embedded (FFPE) tumor samples using the NextSeq or NovaSeq 6000 platforms (Illumina, San Diego, California, USA).13 All variants were detected with >99% confidence based on allele frequency and amplicon coverage, with an average sequencing depth of coverage of >500 and an analytic sensitivity of 5%. ..



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